A High Street retailer is banning cheques at its shops in Sussex and Surrey for a month following the introduction of Chip and Pin.
The month-long cheque ban is being tested at 46 stores
If the trial is successful, shoppers at Boots stores will only be able to pay by cash or card from November.
Shell no longer accepts cheques at its 900 petrol stations and supermarkets Tesco and Asda also restrict their use.
Boots said cheques slowed down till transaction times and were less common since Chip and Pin was brought in.
"We're running this trial to see what our customers think about this change," a spokesman said.
The health and beauty chain has 1,500 stores across the country - 46 of those are in Sussex and Surrey.
The month-long cheque ban is due to begin on 26 September.
The spokesman said the introduction of Chip and Pin earlier in the year had led to a 35% fall in payments by cheque in the first quarter of 2006.
Cheque payments now accounted for two out of every 1,000 purchases, he added.
Halifax said last year that if cheques continued to decline, they could be extinct within two decades.