Sheffield City Airport has lost its licence to operate, meaning no more commercial flights will be allowed.
The licence expired at 2000 BST ahead of formal closure on 30 April, when a business park development begins.
Scheduled flights were cancelled in 2002 but firms have been running pleasure flights and flying lessons from the site.
The Yorkshire Air Ambulance and South Yorkshire Police helicopter will continue to operate on the site.
Peel Airports bought the airport from Sheffield City Council and will turn it into a business park called Blue Skies with a landing strip set aside for helicopters.
Peel Airport has said the site is not making money, blaming the aftermath of the 9/11 attacks in New York for the airport's failure.
However the airport closure has been criticised, with Sheffield City Council and Peel Airports blamed by critics for not doing enough to encourage airlines to move in.
A pressure group - Sheffield City Airport Movement - is taking legal advice to prevent the runway from being dug up.
Millionaire businessman Andrew Cook has also said he would buy the airport to prevent it from being closed down.
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