Police investigating South Yorkshire's oldest unsolved murder have revealed a major breakthrough in the case thanks to new DNA technology.
Police believe the new DNA evidence could lead them to Anne's killer
The naked body of 13-year-old Anne Dunwell was found in a manure heap near Maltby, Rotherham, on 6 May 1964. She had been sexually abused and strangled.
Detectives who recovered DNA from the original exhibits have discovered that it contains "unusual features".
"This is a very exciting development," said Det Sgt Sue Hickman.
She said South Yorkshire Police would now be approaching a number of families in the region and seeking their co-operation to identify relatives from the 1960s.
"It is now possible to identify families on the national DNA database who have this uncommon aspect to their make-up," said Ms Hickman.
The technique is similar to familial DNA, which was successfully used by South Yorkshire Police earlier this year to trace the so-called Dearne Valley Shoe Rapist James Lloyd, who was jailed for life in September.
"This is a very exciting development and I hope that it will finally give Anne's family the closure they deserve," said Ms Hickman.
"Innocent people have nothing to fear from this inquiry as we can conclusively eliminate anyone who is not the offender.
"The only person with anything to fear is the individual who sexually abused and murdered Anne Elizabeth Dunwell."
The teenager had visited her aunt at Bramley on the evening of Wednesday 6 May 1964 and had planned to spend the night there.
But she decided to return home to Whiston to be with her grandmother, who would have been alone as her grandfather was working nights.
She set off from her aunt's that night to catch the bus, but never made it home.
Her body was found the next day, off Carr Lane, between the villages of Carr and Slade Hooton.
A post-mortem examination revealed Anne had been strangled with her own stockings.
Hundreds of officers have since worked on the murder hunt, which was the subject of a cold case review in 2002.
The case has regularly featured on the BBC's Crimewatch programme and a large number of men have already been traced and eliminated from the investigation.
Ms Hickman said: "We're working very closely on this investigation with the Forensic Science Service and the Home Office Police Standards Unit, which are supporting cold cases around the country as part of a national operation.
"Cases like this one will never be closed and will be reviewed with each new development in forensic science - until the culprit is known."