Union members have said they are willing to accept a pay freeze
Nottingham Tram workers have walked out in a row over pay and conditions.
In the latest in a series of strikes services between Hucknall and the city centre will operate on a reduced timetable until 1830 BST on Saturday.
Union leaders said the reluctance of Nottingham Tram Consortium (NCT) to meet to discuss a pay settlement had led to the decision.
NCT said it was still prepared to work with the GMB to find a solution. Tram workers last walked out on 27 July.
A replacement bus service is operating from Phoenix Park to the city centre until 1815 BST.
Anyone travelling to the city's Goose Fair is advised to travel by bus.
The union had originally sought a 5% pay increase but NTC rejected this, saying current economic conditions meant it faced a drop in income.
NTC's offer of a 1% rise was turned down last month by the union's members with the GMB saying the deal had too many strings attached.
The union has since agreed to a pay freeze but the dispute now centres on the failure to reach an agreement over cost savings.