Senior staff from the University of York have been explaining why land to the east of Heslington is the ideal site for a multi-million pound campus.
During the public meeting at St Paul's Church in Heslington, city officials and university representatives faced tough questions about the development.
Concerns were raised about potential traffic congestion and any loss of green belt land.
Opponents of the scheme claim there are other sites more suitable for the plan.
Chair of Heslington parish council, Richard Frost told the meeting that he thought there were other sites around the city that the university should be looking at.
A spokesperson for City of York Council said: "The meeting was just one of three consultation meetings we are holding with members of the public to discuss the plans.
"At this stage no decision on what will happen at the site has been decided."