The new terminal is expected to open in 2014
Heathrow Airport's Terminal 2 is closing after 54 years to make way for a £1bn replacement.
The last passengers checked in to the terminal on Monday, with work due to start on the new building in 2010. It is expected to open in 2014.
BAA chief executive Colin Matthews called the redevelopment an "important moment" in Heathrow's history.
The terminal has handled 316 million passengers since its opening, with 7.5 million passing through last year.
Mr Matthews said: "The closure of Terminal 2 marks an important moment in the modernisation of Heathrow.
"We look forward to the new Terminal 2 providing a better experience for our passengers, with less impact on the environment."
The Beatles, Frank Sinatra, Marilyn Monroe, Elizabeth Taylor and Princess Margaret have all used the terminal.
The final flight to land will be an Air France route from Paris.