Tower Bridge was built between 1886 and 1894
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Tower Bridge will be closed for one week while the 100-year-old structure is revamped.
The London landmark will be closed to traffic in both directions from 0600 BST on Saturday until 8 May while work is carried out on its road.
Specialist work will also be carried out on some of the 100-year-old bridge's timbers.
The area will remain open to pedestrians who will still be able to visit the Tower Bridge exhibition.
A Transport for London spokesman said traffic will be diverted into the congestion charging zone via Southwark Bridge and London Bridge and buses will be diverted via London Bridge.
Drivers will not be charged as long as they follow the diversion.
Owned by the Corporation of London, Tower Bridge was completed in 1894 and carries an average of 40,000 vehicles a day.