Actor Richard Griffiths ordered a woman out of his West End play, after her mobile phone rang for the third time.
The actor has taken direct action against mobile phones before
The performance of Heroes at Wyndham's theatre was nearing the end when Griffiths asked her: "Is that it, or will it be ringing some more?"
The audience clapped as he asked her to leave the Saturday matinee performance.
Last year Griffiths ordered a man out of the National Theatre, when his phone went off for the sixth time during a performance of The History Boys.
Heroes, which also stars John Hurt and Ken Stott, is about war veterans in a nursing home planning their bid for freedom.
It was during the penultimate scene that Griffiths, 58, addressed the woman directly from the stage.
He said: "Could the person whose mobile phone it is please leave?
"The 750 people here would be fully justified in suing you for ruining their afternoon."
The Old Vic's artistic director Kevin Spacey has made a point of embarrassing audience members whose phones go off in the past.
He said: "You have to respect the fact there is some degree of behaviour that we expect in the theatre and we're going to demand it at the Old Vic.
"It's a phone-free zone. We don't want them ringing and we certainly don't want them ringing and people ignoring them pretending that it's not theirs."