He said between 40 and 50 members were involved in the row amid claims that staff had no where to leave their belongings and changing facilities were "appalling".
"We are expecting this move to cause accumulative delays as it could take time to resolve," he said.
Bob Thorogood, general manager of the District line, said: "We understand
that staff have concerns...but taking action in
this way will not resolve the issue.
"All that will be achieved is inconvenience and disruption to customers' journeys.
"We are currently working with the local trade union representatives from both RMT and Aslef.
"We will do our best to provide the best service possible on the District Line and I apologise for any inconvenience that this action will cause."