A convoy of 25 cars and a lorry travelled through London to the mayor's house to protest about the proposed congestions charge zone extension.
The new extended zone could be in place by 2006
Campaigners say enlarging the zone to include Kensington and Chelsea in west London is not necessary as 87% of the roads do not have traffic problems.
The convoy headed from Kensington to Ken Livingstone's house in Cricklewood, north London, on Saturday.
The West London Residents' Association says this is the first of many convoys.
The extension would see the charge zone stop just before Shepherd's Bush to the west, up to Kensal Green Cemetery in the north and down to Chelsea Embankment in the south.
If it goes ahead, it could be in place as early as 2006.
Natalie Cutler, from the association, described the protest as a "dress rehearsal" and said many businesses would be joining the campaign after Christmas.
She told BBC London: "The protest is about the fact that Ken Livingstone is not listening to the people of London.
"We've had a consultation and two thirds of Londoners said they don't want the extension of the charge zone coming to west London."
Ms Cutler said the demonstration would follow a route through the area which would be most severely hit as it has some of the "poorer households".
She also said the smaller businesses that serve the mainly residential areas which would be included in the extension would be damaged.
Ms Cutler added: "Please Mr Livingstone, listen to the people. This is not what Londoners want."
Currently motorists pay £5 to drive into central London between 7am and 6.30pm Monday to Friday.