Bank Tube station will be at the heart of a simulated terrorist strike on London Underground (LU) scheduled to take place next month.
A simulated chemical or biological attack is expected
The police, fire, ambulance and health services will test their responses to an attack on the Tube during the exercise at the City of London station.
The date finally set for the test, delayed earlier this year because of the war in Iraq, is Sunday, 7 September.
The Department for Transport says it will release more details in the coming weeks, but the exercise is expected to test emergency response to a chemical or biological attack on the Tube.
Members of the public will not be involved and the station is likely to be shut for the duration of the exercise.
Tube trains on the six lines which serve the station will not stop at Bank, although they will be allowed to run through to the next station to minimise disruption.
A Department for Transport spokeswoman said: "An exercise will be held at Bank Underground station to allow London's emergency and health services to practise their response to a terrorist attack on the Tube."
"This is one of a number of regular exercises designed to test emergency procedures and equipment carried out in London and across the country.
"It is not a response to a specific threat."
An LU spokeswoman said Bank had been used before for LU's own emergency exercises.
She said: "Bank is normally quite quiet on a Sunday and one part of the station is the Waterloo and City Line which is closed on Sundays.
"The station will be closed but trains will run through so there won't be major disruption to services."