Detectives are hoping advances in forensic testing will help them solve a murder inquiry started in 1969.
Mrs Walker was found battered to death in her living room
Annie Walker, 76, a former pub landlady, was beaten to death at her home in Heather, Leicestershire, by an attacker who also stole £1,000 in cash.
Despite extensive inquiries at the time the killer was never found.
Police have reopened the case file hoping DNA technology will provide the breakthrough needed to catch the victim's killer.
Mrs Walker, who lived alone, was found battered to death in the front room of her house.
She was still wearing her nightclothes and had been battered to death around the head with a blunt edged weapon - which was never found.
She had gone to her bank in Coalville and withdrawn £1,000 from her bank account. The money had disappeared by the time her body was discovered.
It would be worth 10 times that amount today.
The case was dubbed the "Coronation Street Murder" at the time because Mrs Walker's namesake also ran a pub in the soap opera.
It is suspected that Mrs Walker was followed out of the bank where she withdrew the money and to her home, where the murderer struck.
Forensic scientist Hazel Johnson said blood stains on an item of clothing were being re-examined.
"If we feel there are stains that could have originated from someone else we will sample those for DNA profiling," she said.
"Today, if we can go back to that blood stain we should be able to get a DNA profile and come up with a profile of statistics of one in a billion."
Det Chief Insp Mick Mills, who heads the "cold cases" section at Leicestershire Police, said it was not too late to bring the killer to justice.
He said: "Despite the passage of time, Annie still requires justice and she still needs to be put to rest properly. "Whether it's five years or 55 years ago if it's unsolved then we'll keep revisiting and reviewing it to see of there's any further evidence."