Protesters gathered at the Humber Bridge on Saturday to call for tolls at the crossing to be scrapped.
The Humber Bridge is saddled with £333m of debt
Campaign group Humber Action Against Tolls says the charges adversely affect the local economy and punish hospital patients who have to use the bridge.
Group spokeswoman Jenny Walton said: "The tolls are the most expensive in Britain and we want them gone.
"They hurt families and businesses, discourage tourism and damage the economy on both sides of the river."
The protest included representatives of the Cancer Patients Involvement Group which opposes the cost to patients travelling from the south bank of the Humber to hospitals in East Yorkshire for life-saving treatment.
Also taking part in the demonstration were Hull Lib Dem MEP Diana Wallis, cross-party representatives from North Lincolnshire Council, regional business groups and the Road Haulage Association.
In June, the Humber Bridge management team revealed that they had urged Transport Secretary Alistair Darling to agree to a "payment holiday" of several years on the structure's £333m debt.
Bridge master Peter Hill said tolls could then be cut significantly because 80% of its income went on debt payment.