Higher toll fares to cross the Humber Bridge have been approved by the Secretary of State for Transport after a public inquiry into the new charges.
More than six million vehicles cross the bridge every year
Car drivers face a 20p rise to £2.70 each way, while buses will pay an extra 90p, from midnight on 23 April.
And there will be no concessions for hospital patients.
Chairman of the Humber Bridge Board, Councillor David Gemmell, said they could not be expected to underwrite the cost of transporting patients.
He said the health service used to provide transport for patients travelling to the north bank after moving its facilities there but had since stopped.
"They can't then expect us to pick up the tab and thereby underwrite the NHS," he said.
"We can't afford it with £330m of debts."
More than six million vehicles cross the bridge every year, generating approximately £18m.
The Humber Bridge Board pays out £15m every year in repayments. It is estimated the construction loan will be paid off in 2032.