The first unit began treating patients in March 2007
A second mobile chemotherapy unit has been bought to help patients in the three counties of Gloucestershire, Herefordshire and Worcestershire.
Tetbury-based charity Hope For Tomorrow has unveiled the new unit at a fundraising day at Beaufort Polo Club.
The facility is able to treat five patients at a time, and up to 20 a day in the area where they live.
The charity was set up by Christine Mills of Tetbury, whose husband David died from cancer in 2002.
The unit will be run by the oncology team from the Gloucestershire Hospitals NHS Foundation Trust.
The first £150,000 unit began treating chemotherapy patients in Cirencester and Ross-on-Wye in March 2007.