Gloucestershire police say they believe a woman who has been missing from her home in the Forest of Dean for two months has been murdered.
Mrs Prout was last seen at her home in Redmarley on 5 November
Kate Prout, 55, of Redmarley, was last seen on 5 November 2007. She is white, 5ft 6ins tall and of medium build.
Officers originally spent five weeks conducting a search of her home, a 200-acre farm.
Mrs Prout's husband was arrested on suspicion of her murder and later released on bail without charge.
"Despite exhaustive efforts we have unfortunately not discovered any evidence to suggest that Mrs Prout is alive and that she disappeared of her own volition," said a spokesperson for Gloucestershire Police.
"While information may yet come to light to change this view this is, sadly, the case at this point.
"We fear, therefore, that either some harm has befallen her accidentally or that she has been murdered.
"Had the former occurred, we believe it likely that she would have been discovered by now and, as a result, we fear more strongly then ever that she has been murdered."
Mrs Prout's brother, Richard Wakefield, 57 said: "With Kate not having been found during the last agonising two months it was a very difficult Christmas for us.
"We are now having to face a new year not just without her but without any certainty about what has happened to her.
"Discovering the truth, whatever that may be, will put us out of this agony of not knowing, so I appeal once again to anyone who knows anything which could help to come forward and help us by helping the police."