The abbey ruins are adjacent to the restored Forbury Gardens
A Berkshire footpath, leading through an abbey founded in 1121 by King Henry I, will close temporarily while work is carried out on the ruins' masonry.
The public access route through the Cloister Arch of Reading Abbey is to shut following an architect's survey, the borough council said.
Visitors to the ruins - next to Forbury Gardens - can reach Chestnut Walk by following a diversion route.
Access to the chapter house and the treasury will also be closed.
Graeme Hoskin, the borough council's lead councillor for culture and sport, said: "If there is any risk, however small, to the public then it is important that we take these precautions.
"We will now be assessing what works are required in order to make this part of the abbey ruins completely safe and protect them for future generations."
The abbey was once one of the wealthiest in medieval England.