Councillors have rejected plans for a rail freight interchange in Hertfordshire for the second time.
HelioSlough Ltd wanted to build the Strategic Rail Freight Interchange (SRFI) on a 1,035 acre (419 hectare) site on North Orbital Road, St Albans.
St Albans City and District Council rejected the proposal because the development would be in the green belt separating outer London and St Albans.
In 2007, a similar plan for nearby Radlett was turned down.
HelioSlough Ltd, lodged an appeal but in 2008 the Secretary of State ruled the appeal be dismissed and planning permission refused.
A council spokesman said 350 people came to a meeting on Monday night at the Alban Arena, and more than 100 watched the planning referrals committee on the internet.
The committee refused the application for 14 reasons, which included the proposals being on a green belt, the scale of the project and the plans did not show the development could operate in a sustainable way.