Administrators confirmed the players are going without any wages.
Administrators for troubled Darlington FC have said the situation is "precarious" after failing to find a buyer by their deadline.
The club, known as the Quakers, went into administration in February.
Brackenbury Clark and Co set a deadline of 5 May for interested parties but it said no acceptable offers had been received by that time.
Assistant manager Martin Gray and nine of the club's backroom staff have lost their jobs, leaving just five staff.
In a statement, administrator Dave Clark said: "As everyone is aware, we set a deadline of 5 May for all interested parties to contact us in respect of purchasing Darlington Football Club.
"Unfortunately to date there have been no offers for the club that we are able to accept.
"We will continue to search for a purchaser for the club whilst we are able to operate, but the situation is precarious."
He said the club had only been able to operate until the end of the season because of funds provided by former Darlington chairman George Houghton.
Mr Clark said: "Unfortunately yesterday we had the unpleasant task of letting more staff go in order to cut costs, taking the current personnel to the absolute minimum."
He said the remaining staff would continue to defer part of their wages.
Mr Clark said he would continue to run the club for as long as possible but, until a buyer was found, he could not say what the future for the Quakers would be.
Former Darlington vice-chairman Raj Singh made an offer to buy the club, but later withdrew it.