Trustees allocated grants of up to £60,000 to each of 10 projects following a similar announcement 11 days ago.
In March, 100 organisations were invited to apply for help from the fund. Proposals from those remaining will be announced in the coming weeks.
The fund invited 100 organisations to apply for grants
Each charity was asked to submit proposals which would benefit "vulnerable young people", children, "the socially excluded" and "survivors".
The newly announced grants total £532,351 and benefit projects such as:
Aids charity London Lighthouse, in which the princess took a close interest, which has been awarded £60,000.
The St Matthew Society gets £60,000 towards the building of accommodation for vulnerable homeless people in Attleborough, Norfolk.
The Passage, a group refurbishing a building in London's Victoria for homeless over-25s, gets a grant of £60,000.
Refuge, established in 1971 as the world's first safe house for victims of domestic violence, gets £58,736 for counselling.
British Youth Opera gets £50,000 to fund the 1998 Princess of Wales Summer Season.
Gloucestershire County Cricket Club, based in Bristol, gets £53,000 for the construction of a sporting education centre.
Childline gets £52,000 - the cost of keeping one of its phone lines open for a year, including volunteer training, paid counselling supervisors and administration.
Scottish Pre-School Play Association gets £60,000 for its quality assurance accreditation scheme to improve service standards.
Chester Summer Music Festival gets £28,615 to meet an expected deficit in its education outreach programme.
The Albany, based in Lewisham, south-east London, gets £50,000 to support an arts education project for a deprived area of the capital.
Those affected by landmines will share £1m
Six causes of which Diana was either patron or president at the time of her death - Great Ormond Street Hospital for Sick Children, Centrepoint (for homeless youngsters), The Leprosy Mission, the Royal Marsden Hospital (cancer care), National Aids Trust and English National Ballet - have already received grants of about £1m each.
Another £1m has been awarded to the Osteopathic Centre for Children and a further £1m set aside for organisations involved with those affected by landmines.