Shambo first tested positive for bovine TB three months ago and has been at the centre of a legal battle since.
More than 100 monks and pilgrims gathered outside Shambo's enclosure at the west Wales community.
One of the temple monks, Brother Michael, during pooja celebrating the sanctity of life.
The community and supporters started chanting and praying for Shambo early on Thursday morning.
Brother Alex examined paperwork presented by an assembly government vet at the community's gate.
The Hindu monk also spoke to police chief inspector Ian John by the gates of Skanda Vale as the vets were turned away.
Brother Alex met Defra official Wyn Buick who was denied access and left to get a warrant.
When officials returned, they came with equipment to cut their way in.
Police began to carry away protesters ahead of Shambo's removal.
The temple complex's webcam showed officials approaching Shambo in his pen.
The webcam captures the moment Shambo leaves his pen for the last time.