Comic Relief raised £40,236,142 - the biggest total so far. Nadine Coyle and Kimberley Walsh of Girls Aloud arrive to perform alongside the Sugababes.
Red Nose Day funds more than 1,500 projects in Africa such as Kibera slum, Kenya, visited by TV duo Ant McPartlin and Declan Donnelly.
Losers on Comic Relief Does The Apprentice were the boys team, captained by former No 10 spokesman Alastair Campbell.
Fans of Comic Relief Does Fame Academy raised over £795,000. Earlier Kelly Osbourne joined Chris Moyles for his Comic Relief Rallyoke.
Some 60% of the money raised by Comic Relief goes to Africa, 40% is spent in the UK. England spinner Monty Panesar swapped his black patka (turban) for a red one.
More than £10m was raised in the run up to Red Nose Day. PhD students Tom Mitchell and Peter Armitage spent the day taped to Liverpool University.
The Proclaimers teamed up with Peter Kay and Matt Lucas for a Comic Relief version of I'm gonna be (500 miles).