Queues were reported at supermarkets in Spain and in Portugal, where some lorry drivers have launched their own strike.
"We are the ones who move the goods that this country needs to keep working. If we stop because we haven't got the money to buy fuel then the country will stop," said Julio Villascusa, president of Fenadismer.
The hauliers' action follows the lead of British truckers, and Spanish and French fishermen, many of whom have been on strike because of the soaring price of fuel.
Three car manufacturers - Nissan, Mercedes Benz and Seat - said they were suspending factory operations because their supplies of parts had been cut.
There was also very little lorry traffic at Mercamadrid, the main wholesale food market in the Spanish capital, the Associated Press news agency reported.
But the government dismissed fears of shortages, saying it would guarantee market supplies.
It says it hopes to have an assistance package ready to offer the sector within days.
Have you been affected by the Spanish hauliers' strike? Are you a haulier taking part in the strike? Send your comments using the form below, or text 61124 (UK) +44 7624 800 100 (Int):
You can send your pictures and moving footage to firstname.lastname@example.org or text them to 07725 100 100 or short code 61124
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