Academy award-winning director Paul Haggis has recorded the session to create the accompanying video.
The group of pop stars broke into an acapella version of the song Lean On Me during the recording.
Quincy Jones, who produced the 1985 original, announced last week that the song would be re-recorded to help the people of Haiti in the wake of the earthquake last month.
The song was written by Michael Jackson and Lionel Richie.
Other artists singing on the new track include Carlos Santana, Jennifer Hudson, Natalie Cole, Justin Bieber, Brian Wilson, Miley Cyrus, Usher, Enrique Iglesias, Josh Groban, Harry Connick Jr, Akon, Celine Dion, Snoop Dogg and Tony Bennett.
Quincy Jones and Lionel Richie reunite for the 2010 version
None of the original performers returned for the re-recording.
Lil' Wayne said he had friends in Miami who had lost relatives in the disaster.
"I think it's amazing what's been done for Haiti, but I think it's amazing what hasn't been done for New Orleans," he said.
Money raised from the original single - which topped $30m - went to USA for Africa to fund hunger relief in Africa.
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