Superman's cape, Indiana Jones' whip, John Lennon's jacket and Elizabeth Taylor's wig are among 850 "pop culture" items to be sold next month.
The collection includes a Superman costume worn by Christopher Reeve
The collection, owned by US property developer Anthony Pugliese, has been valued at more than $5m (£2.5m).
Other lots in the Las Vegas auction will include a wedding dress worn by Madonna in her Like a Virgin video.
The pointed hat worn by the Wicked Witch of the West in The Wizard of Oz will also be sold.
"This collection is as good as it gets," said Arlan Ettinger, president of the Guernsey's auction house.
Christopher Reeve's costume from the first two Superman films has an estimated price tag of $60,000-$90,000 (£30,200-£45,300).
Harrison Ford's bullwhip from the first three Indiana Jones films is expected to fetch $50,000-$75,000 (£25,200-£37,700).
The brown velvet jacket worn by John Lennon in the Imagine video has an estimated value of $50,000-$60,000.
The Egyptian wig worn by Elizabeth Taylor in her 1963 film Cleopatra, a resin statuette from The Maltese Falcon and a sword used in Braveheart are among the movie props in the collection.
Non-film related items include the gun Jack Ruby used to shoot President Kennedy's assassin Lee Harvey Oswald and the toe tag used to identify his corpse.
"Most collections have one or two great things and then things trail off from there," Mr Ettinger said.
"This collection, there would have to be 100 items here, any one of which would be a star attraction anywhere else."
The items will go under the hammer at the Palms Resort and Casino on 15 and 16 March.