The money will be given to the Indian charity City of Joy Aid
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The black dress worn by Audrey Hepburn in Breakfast at Tiffany's is to be auctioned for a charity which helps under-privileged people in India.
The outfit was designed by Hubert de Givenchy, who was in charge of Hepburn's wardrobe in the 1961 film.
The actress played Holly Golightly and is seen in the dress in the opening scene as she emerges from a taxi.
It is expected to fetch between £50,000 and £70,000 when sold by Christie's in London on 5 December.
Hepburn was born in Brussels in 1929, and died from colon cancer at the age of 63.
She had spent her later life concentrating on humanitarian work, including a role as a goodwill ambassador for Unicef, which began in 1989.
Breakfast at Tiffany's was adapted from a novel by Truman Capote and also starred George Peppard, Patricia Neal and Mickey Rooney.