Brokeback Mountain has been beaten by Crash to the main prize at this year's Screen Actors Guild awards in LA.
Cast members of Crash, including Matt Dillon and Sandra Bullock (left)
Racial drama Crash won the prestigious best ensemble cast prize - the equivalent of a best film award.
Capote's Philip Seymour Hoffman and Walk The Line's Reese Witherspoon won best actor and actress. UK star Rachel Weisz took best supporting actress.
The SAG awards are part of the raft of film awards building up to the Oscars. The nominations are out on Tuesday.
Ang Lee's cowboy romance Brokeback Mountain, starring Heath Ledger and Jake Gyllenhaal, has so far dominated many of the pre-Oscars award ceremonies.
Witherspoon, who won her SAG award for her role in Johnny Cash biopic Walk The Line, said: "Sometimes, I can't just shake the feeling that I'm just a little girl from Tennessee."
She won the award for best performance by an actress in a musical or comedy at the Golden Globes earlier this month.
She added that her character, singer June Carter - who married country star Johnny Cash - was her "biggest inspiration".
Hoffman, seen by many as one of the favourites to win the best actor Oscar for his performance in Capote, about the life of American writer Truman Capote.
"It's important to say that actors can't act alone, it's impossible. What we have to do is support each other," he said.
Hoffman and Weisz - who was honoured for her role in The Constant Gardener - were also winners of Golden Globe awards.
"It's so special to be honored by fellow actors, so thanks very much to the tribe," said Weisz on collecting her award.
Paul Giamatti, who won best supporting actor for his part in boxing drama The Cinderella Man, said: "I can't imagine a greater honour than being acknowledged by my peers."
Last year, the wine-country comedy Sideways, which co-starred Giamatti, won SAG's ensemble prize, while Million Dollar Baby went on to earn best film at the Oscars.
In the awards for television Felicity Huffman won best comedy actress for her small screen role in Desperate Housewives, which also won best ensemble prize.
Lost won best ensemble prize for a TV drama, while Kiefer Sutherland was named best actor for his role in 24.