The late Johnny Cash's video for his song Hurt has come top of a list compiled by a panel of music artists and industry insiders.
Cash died in September 2003 after complications from diabetes
The top 20 list was commissioned by mobile phone company 3 to celebrate over three decades of pop music videos.
Michael Jackson's 1983 video for Thriller came second, followed by Aphex Twin's Come To Daddy from 1997.
Panellists included musicians Norman Cook and Michael Stipe along with Brit Award front-runners Franz Ferdinand.
REM lead singer Michael Stipe said of the number one video: "Just heartbreaking. Kudos to director Mark Romanek for having the audacity and the courage to do a video like that."
"The video is just devastating. And beautiful. It might be an obvious choice, but it touched me in a really big way."
TOP FIVE MUSIC VIDEOS
1. Hurt - Johnny Cash
2. Thriller - Michael Jackson
3. Come To Daddy - Aphex Twin
4. Just - Radiohead
5. Billie Jean - Michael Jackson
Source: 3 poll
Tom Fletcher, singer with teenage pop band McFly, chose Michael Jackson's Thriller as his best video.
"A good video shows a band's personality and should make the song better. It should be able to stand on its own," he said.
Completing the top five was the 1995 Radiohead video for the song Just at number four, followed by a second entry for Michael Jackson with Billie Jean at number five.
Queen's Bohemian Rhapsody video made it to at number 18, whilst Madonna's only entry was for the 1990 song Vogue reached number ten.
Other members of the panel included current artists Keane, Jamie Cullum and Amy Winehouse alongside The Cure frontman Robert Smith and pop diva Bjork.