The famous talking car from 80s TV show Knight Rider will go under the hammer on Friday, along with a Ferrari driven by Tom Selleck in 80s series Magnum PI.
David Hasselhoff starred in Knight Rider
The Knight Rider car, named Kitt, is expected to fetch between £22,000 and £33,000 when it goes up for auction on website eBay on Friday.
The 1979 Ferrari is estimated to make between £32,000 and £43,000.
Two jackets worn by Marlon Brando, who died earlier this month, and a Marilyn Monroe dress are also on offer.
A pinstriped suit coat worn in 1955's musical Guys and Dolls is expected to fetch between $4,000 (£2,200) and $6,000 (£3,250).
Brando starred in more than 40 movies
A sports jacket Brando donned in spy film Morituri (1965) is estimated to fetch up to $1,500 (£800).
Brando, the Oscar-winning star of The Godfather and Apocalypse Now, died on 2 July 2004, aged 80.
Joseph Maddelena, who owns US auction house Profiles in History, said the jackets were due to be sold before Brando's death earlier this month.
But Mr Maddelena said he did not expect the jackets to fetch more now that the actor was dead.
"I don't think it's going to make that big of a difference," he said.
The Kitt car was voted the UK's second most favourite on-screen vehicle in a recent survey by website Yahoo!
It was beaten by the Lotus Esprit from James Bond film The Spy Who