Eight former executives of the largest insurance broker in the US, Marsh & McLennan, have been indicted on charges involving price-rigging.
Mr Spitzer is known as a force to be reckoned with
They were accused of colluding with executives at major insurance firms to arrange non-competitive bids, said New York Attorney General Eliot Spitzer.
The practice meant that corporate clients were denied the best prices for the policies they required, he said.
All of the eight defendants pleaded not guilty to the charges.
The case is part of Mr Spitzer's widening investigation into the insurance industry.
'No responsible argument'
Mr Spitzer's crusades against corporate sleaze have earned him the nickname "Sheriff of Wall Street".
The attorney general charged William Gilman - alleged to be the architect of the price-rigging scandal - and seven other former senior executives at Marsh with 37 counts, including scheming to defraud and grand larceny.
"These are very senior executives within the Marsh hierarchy," said Mr Spitzer.
"Not only was it wrong, it was harmful to the economy. There is simply no responsible argument in favour of rigging bids, stifling competition and cheating."
If convicted, the defendants could face sentences of between 15 and 25 years in prison.
The price rigging is believed to have cost Marsh & McLennan's corporate customers millions of dollars.
On Thursday, shares in Marsh & McLennan were down 15 cents at $28.73 in New York.