The BBC's Justin Webb in Washington says Mr Obama's public reiteration of his view - a day after his Secretary of State Hillary Clinton had laid it out only to have it shot down by the Israeli government - has raised eyebrows in Washington.
Ahead of his visit to the Middle East next week, Mr Obama has put Mr Netanyahu on notice that this White House has a firm agenda of its own, our correspondent adds.
The White House meeting between the two leaders is part of an effort by the Obama administration to restart stalled peace talks.
Mr Obama has already met King Abdullah of Jordan and Mr Netanyahu. He plans to meet Egyptian President Hosni Mubarak in Cairo on 4 June.
Earlier on Thursday, Mrs Clinton said Washington was pushing for a two-state solution in the Middle East as it was in the "best interests" of both the Palestinians and Israelis.
Speaking after a dinner with Mr Abbas, she said: "We believe strongly in a two-state solution."
However, Israeli government spokesman Mark Regev said on Thursday that Israel would continue to allow some construction in West Bank settlements despite US calls for a freeze on its work.
He said the fate of the settlements should be decided in peace talks with the Palestinians.
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